bdca1 apc  (Miltenyi Biotec)

Journal: Cancer Cell

Article Title: Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NECTIN2-TIGIT axis as a target for immunotherapy

doi: 10.1016/j.ccell.2023.12.008

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Article Snippet: Mouse Anti-CD1c APC-R700 , BD Biosciences , Cat# 566615; RRID: AB_2869795.

Techniques: Recombinant, Modification, Staining, RNA Sequencing Assay, Transduction, Software

Journal: iScience

Article Title: Human skin CD141 + dendritic cells regulate cutaneous immunity via the neuropeptide urocortin 2

doi: 10.1016/j.isci.2023.108029

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Article Snippet: Mouse monoclonal anti-human CD1c/BDCA1-PE/APC (Clone: AD5-8E7) , Miltenyi Biotec , Cat # 130-113-302.

Techniques: Purification, Recombinant, Staining, Concentration Assay, Clinical Proteomics, Sterility, cDNA Synthesis, Reverse Transcription, Multiplex Assay, Labeling, Expressing, Transfection, Software

Journal: Scientific Reports

Article Title: CD1b glycoprotein, a crucial marker of thymocyte development during T cell maturation in cynomolgus monkeys

doi: 10.1038/s41598-023-41708-y

Figure Lengend Snippet: ( a ) Histograms showing expression of CD1a, CD1b, CD1c, and CD1d by total thymocytes. ( b ) Dot plot showing the five developmental stages, defined according to expression of CD3 and CD1b, and a graph showing the percentage of cells at each developmental stage. ( c–g ) Dot plots showing CD4/CD8 expression patterns at each developmental stage. The red box in panel d denotes the ISP stage, and the red box in panel f denotes the CD4 hi CD8 lo intermediate stage. ( h ) Dot plots and graphs showing expression of intracellular TCR Vβ and CD28 according to developmental stage (up to CD3 lo CD1b + DP stage). ( i ) Dot plots showing CD4/CD8 expression by CD3 hi thymocytes. ( j ) A contour plot showing CD1b + and CD1b − thymocytes among CD3 hi CD4/CD8 SP thymocytes. ( k ) A contour plot showing CD1b + and CD1b − thymocytes among CD3 hi DP thymocytes and a histogram comparing CD1b expression levels by DP thymocytes at each developmental stage. This histogram uses the modal option to scale all channels as a percentage of the maximum count. ( l ) A schematic diagram showing the order of the developmental stages of thymocytes, based on the expression of CD1b. Each number indicates the following stage: (I) CD3 - CD1b - DN, (II) CD3 - CD1b + DN, (III) CD3 - CD1b + ISP, (IV) CD3 - CD1b + DP and CD3 lo CD1b + DP, (V) CD3 hi CD1b + DP, (VI) CD3 hi CD1b + SP, mCD4 SP: CD3 + CD1b - mature CD4 SP, mCD8 SP: CD3 + CD1b - mature CD8 SP, mDP: CD3 + CD1b - mature DP. Data are representative of six independent experiments, each with a similar result. Data are expressed as the mean ± SD (n = 6). * p < 0.05; ** p < 0.01. i.c.: intracellular.

Article Snippet: The following fluorochrome-labeled human monoclonal antibodies were used for flow cytometry analysis: CD3-PerCP-CyTM5.5 (SP34-2, BD Biosciences), CD4-PE/Cyanine7 (OKT4, BioLegend), CD8α-Brilliant Violet 711TM (SK1, BioLegend), CD8β- PE/Cyanine7 (SIDI8BEE, eBioscience), CD1a-FITC (NA1/34-HLK, Invitrogen), CD1b-APC (SN13, BioLegend), CD1c-APC (L161, BioLegend), CD1d-Brilliant Violet 421TM (51.1, BioLegend), CD11b-PE (ICRF44, BD Biosciences), CD11c-PE (3.9, BioLegend), CD14-PE (M5E2, BD Biosciences), CD20-PE (2H7, BioLegend), CD28-PE (CD28.2, BD Biosciences), CD31-Brilliant Violet 421TM (WM59, BioLegend), CD69 (FN50, BioLegend), HLA-ABC (G46-2.6, BD Biosciences), HLA-DR, DP, DQ (Tü39, BioLegend), CCR7-PE (G043H7, BioLegend), CXCR3-PE/Cyanine7 (G025H7, BioLegend), CD95-Brilliant Violet 510TM (DX2, BioLegend), CD159a (NKG2AC)-APC (Z199, Beckman Coulter), TCR Vβ F1- PE/Cyanine7 (8AC, Invitrogen), TCR Vα24-Jα18- APC/Cyanine7 (6B11, BioLegend), TCR Vα7.2-APC/Cyanine7 (3C10, BioLegend), PLZF-PE (Mags.21F7, eBioscience), EOMES-FITC (WD1928, eBioscience), and IL-4-Brilliant Violet 421TM (MP4-25D2, BioLegend).

Techniques: Expressing